Publications

Learn more about Deterministic Cell Separation™ (DCS) and the Curate® Cell Processing System

Publications

Deterministic Cell Separation recovers >2-fold T cells, and more naïve T cells, for autologous cell therapy as compared to centrifugally prepared cells.

ASH Poster December 2021

DCS technology enables a breakthrough in producing T cell therapies, recovering more total and naive T cells with greater purity. This approach ensures minimal manipulation, significantly streamlined workflows, reduced need for skilled technician time, while simultaneously improving cell recovery and purity of leukocytes for apheresis products.

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Deterministic Lateral Displacement – The next generation of CAR-T cell processing?

SLAS TECHNOLOGY: Translating Life Sciences Innovation, January 2018

Demonstration of 80% cell recovery and 87% platelet depletion from apheresis blood products processed with DLD technology. DLD T-cell preparations showed a high conversion to the T-central memory (Tcm) phenotype and expanded well in culture, resulting in 2-fold greater Tcm compared to Ficoll and direct magnetic approaches. DLD processing provides a path to develop a simple, closed, and automated system for addressing multiple processing steps, human error, contamination, and other technical challenges when manufacturing of therapeutic cells.

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Automated Leukocyte Processing by Microfluidic Deterministic Lateral Displacement

Cytometry Part A, 89A: 1073-1083, December 2016

Presents the reduction-to-practice of DLD technology with a commercially produced, high precision plastic microfluidic chip-based device designed for automated and simultaneous washing and size-based separation of human leukocytes, without centrifugation or manual handling of blood samples. The developed protocol averaged 88% WBC recovery, >99% removal, and >99% removal of unbound antibody in ~18 min for 200mL samples.

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Sample Preparation for Flow Cytometry Benefits From Some Lateral Thinking

Cytometry Part A, 89A: 1054-1056, November 2016

Review of DLD technology as an improved sample preparation method for flow cytometry, and highlighting how DLD processing can remove both RBCs and excess antibody without the need for lysing agents or centrifugation while eluting the target cells in an appropriate volume for rapid flow cytometry-based analysis.

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Automated Processing of Human Blood Leukocytes by a Plastic Disposable Microfluidic Deterministic Lateral Displacement Device

CYTO Poster June 2016

Demonstration of improved RBC removal and unbiased WBC recovery from human whole blood samples with minimal hands-on time and no adverse affects on cell viability when using reduced-to-practice DLD technology in plastic microchip format.

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DLD Microfluidic Purification and Characterization of Intact and Viable Circulating Tumor Cells in Peripheral Blood

AACR Poster April 2016

A polymer-based chip-to-chip approach to purify circulating tumor cells. Microfluidic methods using DLD technology provide a uniquely effective and gentle way of processing cells. An example, presented here, being the isolation of CTCs from 1 in a billion blood cells from Breast Cancer Patients.

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Inhibition of clot formation in deterministic lateral displacement arrays for processing large volumes of blood for rare cell capture

Lab on a Chip, 10.1039/c4lc01409j, March 2015

Demonstration of optimized DLD (single array) processing for whole blood samples resulting in high yield of harvested PC3 cancer cells. Processing larger blood volumes within one hour is achievable with additional DLD arrays on a single chip.

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Deterministic separation of cancer cells from blood at 10 mL/min

AIP Advances 2, 042107-1-7, October 2012

Presents how a microfluidic DLD device can isolate CTCs from blood with capture efficiencies >85% and at flow rate up to 10 mL/min with no impact on cell viability, enabling the option for downstream cell culture and analysis.

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Improved Performance of Deterministic Lateral Displacement Arrays with Triangular Posts

Microfluidics and Nanofluidics 9, pp. 1143-1149, May 2010

Demonstration of how an optimized DLD array post geometry reduces clogging, lowers hydrostatic pressure requirements, and increases the range of size-based displacement characteristic.

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A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women

Prenatal Diagnosis, 28:892-899, October 2008

Report on the development of a high-throughput and highly efficient microfluidic device for isolating rare nucleated red blood cells from maternal blood.

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Crossing microfluidic streamlines to lyse, label and wash cells

Lab on a Chip, 8:1448-53, September 2008

Demonstration of on-chip cell treatments, such as labeling and washing, and bacterial lysis and chromosomal extraction, using DLD processing.

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